Antisense oligonucleotides that contain DNA are able to form RNA-DNA hybrids. These hybrids can act as a substrate for RNase H, which promotes cleavage of the mRNA target. Because the RNA is degraded, any sequence within the coding region of the target gene has the potential to be a useful antisense site.The goal of the antisense approach is the downregulation of a molecular target, usually achieved by induction of RNase H endonuclease activity that cleaves the RNA-DNA heteroduplex with a significant reduction of the target gene translation (Figure 1). Antisense oligonucleotides (ASOs) are short synthetic stretches of DNA that hybridize with specific mRNA strands that correspond to target genes. Because ribosomes cannot translate double-stranded RNA, the translation of a given mRNA can be inhibited by a segment of its complementary sequence, the corresponding antisense RNA. Alteration of DNA and chromatin. Natural antisense transcripts have been proposed to cause DNA methylation 26, DNA demethylation 33 and chromatin modifications of non-imprinted autosomal loci 27,34.
An antisense is a molecule which interacts with a complementary strand of nucleic acids, so as to suppress its transcription.
However, RNA can form duplexes just as DNA does. All that is needed is a second strand of RNA whose sequence of bases is complementary to the first strand. Example. 5´ C A U G 3´ mRNA 3´ G U A C 5´ Antisense RNA. The second strand is called the antisense strand because its sequence of nucleotides is the complement of message sense.
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what is antisense dna
